What is DHFR selection system?

What is DHFR selection system?

DHFR is used as a selection marker for cells that have successfully integrated the vector. Thus, CHO cells that have successfully integrated the expression vector for the drug of interest will have also integrated the dhfr gene, and will survive in medium deficient in GHT.

What is DHFR gene?

DHFR (Dihydrofolate Reductase) is a Protein Coding gene. Diseases associated with DHFR include Megaloblastic Anemia Due To Dihydrofolate Reductase Deficiency and Megaloblastic Anemia. Among its related pathways are Nucleotide Metabolism and One carbon pool by folate.

Is DHFR a recombinant protein?

Recombinant Human DHFR Protein, CF Summary Measured by the reduction of dihydrofolic acid (DHF). The specific activity is >5,500 pmol/min/μg, as measured under the described conditions.

What is GS CHO cell?

CHO-K1SV is a suspension, protein-free-adapted CHO-K1-derived cell line employing the glutamine synthetase (GS) gene expression system (GS-CHO expression system). The selection of high-producing mammalian cell lines is a crucial step in process development for the production of therapeutic antibodies.

Why is DHFR important?

Dihydrofolate Reductase (DHFR) is a very important enzyme because it produces cofactors that are necessary for DNA synthesis. Specifically, DHFR catalyzes the reduction of folate and 7,8-dihydrofolate (DHF) to 5,6,7,8-tetrahydrofolate (THF).

Where is DHFR located in the cell?

In humans, the DHFR enzyme is encoded by the DHFR gene. It is found in the q11→q22 region of chromosome 5.

What can DHFR be used for?

DHFR can be targeted in the treatment of cancer and as a potential target against bacterial infections. DHFR is responsible for the levels of tetrahydrofolate in a cell, and the inhibition of DHFR can limit the growth and proliferation of cells that are characteristic of cancer and bacterial infections.

Are proteins expressed?

Protein expression also refers to processes which describe how living cells or organisms synthesize, modify and regulate proteins. In protein research, protein expression can now also refer to the use of laboratory techniques required for the manufacture of proteins.

What is the function of DHFR?

Dihydrofolate reductase (DHFR) is a ubiquitous enzyme, which plays an essential role in the biosynthesis of tetrahydrofolate cofactors. It catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate (see Fig. 1 under comments).