How much RNA is in a Bioanalyzer?
How much RNA is in a Bioanalyzer?
Minimal sample consumption is required with only 5 ng of total RNA needed for an effective analysis.
What is Bioanalyzer used for?
The Bioanalyzer can be used to assess the size and quality of DNA over a range of sizes and concentrations. The DNA 1000 assay can be used with extracted, clean DNA to look at the size profile of a sample from 25bp to 1000bp at a concentration of 0.1-50ng/ul.
How much does a 2100 Bioanalyzer cost?
Marshall Scientific’s Recertification Protocol
| Agilent 2100 Bioanalyzer G2939A with Chip Priming Station | Caliper Life Sciences LabChip XT Fractionation System | Agilent 2100 Bioanalyzer G2938B with Chip Priming Station |
|---|---|---|
| Our Price: $10,955.00 | Our Price: $4,055.00 Deal of the Month Price: $3,495.00 | Our Price: $10,145.00 |
What is the difference between Bioanalyzer and TapeStation?
A: The Agilent 2200 TapeStation system can run anywhere from 1 to 95 samples. The Bioanalyzer is “chip- based” and can only fit 11 or 12 samples per run. Therefore, the service fee for Bioanalyzer run is charged on a per chip basis. Both Bioanalyzer and TapeStation can run cDNA or NextGen library samples.
How is Rin calculated?
RIN for a sample is computed using several characteristics of an RNA electropherogram trace, with the first two listed below being most significant. RIN assigns an electropherogram a value of 1 to 10, with 10 being the least degraded. The fast region is the area between the 18S and 5S rRNA peaks on an electropherogram.
How does RNA Bioanalyzer work?
The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA and RNA. The fluorescent dye molecules intercalate into DNA or RNA strands. They are then detected by their fluorescence and translated into gel-like images (bands) and electropherograms (peaks).
How much is a Bioanalyzer?
Agilent 2100 Bioanalyzer Pricing (TBD)
| Assay Kit | Agilent Cat. # | 2017 Price per Chip |
|---|---|---|
| DNA 1000 Kit | 5067-1504 | $36.00 |
| DNA 7500 Kit | 5067-1506 | $36.00 |
| DNA 12000 Kit | 5067-1508 | $36.00 |
| Agilent High Sensitivity DNA Kit | 5067-4626 | $60.00 |
How much is Agilent Bioanalyzer?
How much does a TapeStation cost?
| Harvard Internal | Commercial | |
|---|---|---|
| Service | Setup Fee | Per Sample |
| Agilent TapeStation D1000 | $12.67 | $6.86 |
| Agilent TapeStation Genomic DNA | $13.80 | $8.54 |
| Agilent TapeStation High Sensitivity D1000 | $14.62 | $8.37 |
What is RNA TapeStation?
An Automated Electrophoresis Solution that Delivers DNA and RNA Sample QC. The Agilent TapeStation system is an automated electrophoresis solution for the sample quality control of DNA and RNA samples. It is suitable to analyze size, quantity, and integrity of your samples.
What is a good RIN value for RNA?
High-quality RNA will contain an RIN of at least 8, where partially fragmented RNA will contain an RIN within the range of 6–8. Any RNA sample that has a RIN below 5 should not be subjected to further fragmentation during the ScriptSeq protocol, as it will generate smaller than desired fragments.
How can I improve my RIN RNA?
How to improve RNA Integrity?
- Remove media, wash cells with cold PBS, then add 5 mL TRIzol.
- Incubate samples for 5 min at RT.
- Centrifuge at 5,000 rpm at 4 degree C for 30 min.
- Transfer clear upper phase into new tube.
- Precipitate RNA by adding 2.5 mL isopropanol.
