Do macrophages express CD11b?
Do macrophages express CD11b?
Similarly, CD11b has often been used as a marker for all myeloid cell types. However, some myeloid cell subpopulations are negative for CD11b expression, including alveolar macrophage and CD103+ dendritic cells, while some non-myeloid cell types, such as mature NK cells, express CD11b [8, 10].
Do dendritic cells express CD11b?
CD11b is considered a pan-myeloid marker (expressed after granulocyte-monocyte progenitors (GMP) phase in the bone marrow). CD11c is a prefered marker for dendritic cells. In general, mouse DCs express CD11c but are negative for CD11b.
Do macrophages express CD103?
CD103+CD11b+ lamina propria DCs express low amounts of the macrophage markers CD172a, F4/80 and CX3CR1 and low Csf-1R. In contrast, lamina propria CD103− CD11b+ cells express the macrophage markers CD172a, F4/80, CX3CR1, and the Csf-1R.
What is the link between macrophages and dendritic cells?
Until recently they have been regarded as relatively discrete cell types, with macrophages being a key com- ponent of the innate immune system while dendritic cells interface with the adaptive immune system and modulate immune responses.
Are macrophages CD11c positive?
M1 macrophages were identified as F4/80-positive/CD11c-positive/CD206-negative and M2 macrophages were identified as F4/80-positive/CD11c-negative/CD206-positive.
Are neutrophils CD11b positive?
The inflammatory monocyte subset are Ly6C(hi), neutrophils and eosinophils are Ly6C(int), and the ‘patrolling’ monocyte subset is Ly6C(lo). Therefore CD11b+Gr-1+ cells will include monocytes, neutrophils, and eosinophils. CD11b+Gr-1- will include Ly6C(lo) monocytes.
How are dendritic cells identified?
Overview of Dendritic Cells in the Immune System Physiology. Human DC are identified by their high expression of major histocompatibility complex (MHC) class II molecules (MHC-II) and of CD11c, both of which are found on other cells, like lymphocytes, monocytes and macrophages (5–12).
Where are plasmacytoid dendritic cells found?
Plasmacytoid dendritic cells (pDCs) resemble antibody-secreting plasma cells and are believed to arise from a lymphoid progenitor. pDCs are found in blood and in lymphoid tissues such as lymph nodes, tonsils, spleen, thymus, and Peyer’s patches.
What is the role of macrophages and dendritic cells?
Intestinal macrophages and dendritic cells (DCs) comprise a unique group of tissue immune cells that are ideally situated at the interface of the host and the enteric luminal environment to appropriately respond to microbes and ingested stimuli.
Do dendritic cells come from macrophages?
Classifying mouse dendritic cells We propose that DCs should be classified as a separate lineage of mononuclear phagocytes on the basis of the fact that they arise from adult haematopoietic stem cell (HSC)-derived precursors that are distinct from the precursors of monocytes and macrophages (Fig. 1).
What is the function of the dendritic cell CD103?
The CD103 +CD11b − classical dendritic cell subset. The CD103 +CD11b − cDC subset (referred to here as CD103 + cDCs) shares its origin and function with lymphoid tissue CD8 + cDCs (12, 30). CD103 + cDCs populate most connective tissues. The proportion of CD103 + cDCs among total cDCs rarely exceeds 20–30%.
What makes up the CD11b + CDC subset?
The CD11b + cDC subset consists of a mixture of tissue cDCs and macrophages, which contributed to the confusion that still exists on the exact contribution of DCs and macrophages to tissue immunity. CD11b + cDCs most often lack the integrin CD103 and express the integrin CD11b (Table 1).
How does CD103 + CD11b − DCS repress inflammation?
The CD103 + CD11b − DC subset has gained a functional specialization that able them to repress inflammation via several epithelial interferon-γ (IFN-γ)-induced proteins. Among others, we identified that epithelial IDO1 and interleukin-18-binding protein (IL-18bp) were strongly modulated by CD103 + CD11b − DCs.
What happens to CD103 + CD11b + DCS in DTR mice?
We show that Clec9A–diphtheria toxin receptor (DTR) mice after depletion of CD103 + CD11b − DCs developed severe, low-dose dextran sodium sulfate (DSS)-induced colitis, whereas the lack of CD103 + CD11b + DCs in Clec4a4-DTR mice did not exacerbate intestinal inflammation.
