How is pIC50 calculated?
How is pIC50 calculated?
Simply stated, pIC50 is the negative log of the IC50 value when converted to molar. For example, an IC50 of 1 uM is 1 x10^-6 M and that’s equal to a pIC50 of 6. An IC50 of a nanomolar compound is 1 x10^-9 M, which is a pIC50 of 9.
How do you convert pIC50 to IC50?
An IC50 of 1 nM is 10-9 M, which is pIC50 = 9.0. An IC50 of 10 nM is 10-8 M, which is pIC50 = 8.0. An IC50 of 100 nM is 10-7 M, which is pIC50 = 7.0. An IC50 of 30 nM is 3 x10-8 M, which is also 10-7.5 M, which is pIC50 = 7.5.
What is IC50 value?
Half-maximal inhibitory concentration (IC50) is the most widely used and informative measure of a drug’s efficacy. It indicates how much drug is needed to inhibit a biological process by half, thus providing a measure of potency of an antagonist drug in pharmacological research.
What is the difference between EC50 and IC50?
The EC50 is the concentration of a drug that gives half-maximal response. The IC50 is the concentration of an inhibitor where the response (or binding) is reduced by half.
How is pEC50 calculated?
The pEC50 is defined as the negative logarithm of the EC50. If the EC50 equals 1 micromolar (10-6 molar), the log(EC50) is -6 and the pEC50 is 6. The advantage to expressing potency this way is that drugs with larger pEC50 values are more potent.
What is nanomolar concentration?
Molar concentration (also called molarity, amount concentration or substance concentration) is a measure of the concentration of a chemical species, in particular of a solute in a solution, in terms of amount of substance per unit volume of solution.
How is Dpph IC50 calculated?
TEAC was calculated as follows: TEAC = IC50 of Trolox (µg mL–1)/IC50 of sample (µg mL–1). The higher TEAC value means the higher DPPH radical scavenging activity. In this study, the values of the IC50 of an analytical sample and IC50 of Trolox, measured on the same day, were used to obtain the TEAC.
Why is a low IC50 value advantageous for a drug?
Low IC50 value means that the drug is effective at low concentrations, and thus will show lower systemic toxicity when administered to the patient. This will help experimentalists to better determine drug dosage for future in vivo experiments and clinical trials.
What is a good IC50?
In most cases, the IC50 of the best candidate compound should be lower to 10 micromolar, acceptable for NIH to screen the NCI60 program.
How do you calculate drug KD?
KD is determined experimentally and is a measure of the affinity of a drug for a receptor. More simply, the strength of the ligand–receptor interaction. To determine KD, a fixed mass of membranes (with receptor) are incubated with increasing concentrations of a radioligand until saturation occurs.
Which is greater a pIC50 or an IC50?
Simply stated, pIC50 is the negative log of the IC50 value when converted to molar. For example, an IC50 of 1 uM is 1 x10^-6 M and that’s equal to a pIC50 of 6. An IC50 of a nanomolar compound is 1 x10^-9 M, which is a pIC50 of 9.
How to calculate the IC50 value in Python?
Very Simple IC50 Tool Kit – calculate, measure, determine IC50 online (Python version). Obtain IC50, EC50, etc, by online curve-fitting and get publication-quality plots! Paste in the X and Y values here.
How can I calculate the geometric mean of my IC50?
However, you can easily calculate the geometric mean of your IC50 values if you use pIC50s. This is because the geometric mean is also the arithmetic means of the logs of the IC50 values. Keep this logarithmic thinking in mind when you select your compound concentrations for your dose response curves.
Which is the negative log of the IC50 value?
Simply stated, pIC50 is the negative log of the IC50 value when converted to molar. For example, an IC50 of 1 uM is 1 x10^-6 M and that’s equal to a pIC50 of 6.
