What are cell lines PPT?

What are cell lines PPT?

Continuous Cell Lines or Immortal cell lines  When a finite cell line undergoes transformation and acquires the ability to divide indefinitely, it becomes a continuous cell line.  The continuous cell lines are transformed, immortal and tumorigenic.

What are cell lines in cell culture?

Cell Line. A cell line is a permanently established cell culture that will proliferate indefinitely given appropriate fresh medium and space. Lines differ from cell strains in that they become immortalized.

What is cell line designation?

Nomenclature of Cell Lines: It is a common practice to give codes or designations to cell lines for their identification. For instance, the code NHB 2-1 represents the cell line from normal human brain, followed by cell strain (or cell line number) 2 and clone number 1.

What is cell immortalization?

For the most part, viral genes achieve immortalization by inactivating the tumor suppressor genes (p53, Rb, and others) that can induce a replicative senescent state in cells. Recent studies have also shown that SV40 T antigen can induce Telomerase activity in the infected cells.

What are the characteristics of cell lines?

These properties include morphology, growth characteristics, expression of surface glycoproteins, partial expression of neuroendocrine cell markers, frequent chromosomal evidence of gene amplification, and occasional amplification of the c-myc proto-oncogene.

What is Totipotency PPT?

Totipotency is the ability of a single cell to divide and produce all of the differentiated cells in an organism. In other words, totipotency is the genetic potential of a plant cell to produce the entire plant.

What are the types of cell culture?

There are three major types of cell culture, which include:

• Primary cell culture.
• Secondary cell culture, and.
• Cell line.

What is the difference between primary cells and cell lines?

Primary cell culture is the culture of cells directly isolated from parental tissue of interest; whereas cell line is the culture of cells originated from a primary cell culture, which is generally used to expand cell population and prolong life span.

How are cell lines used in medical research?

For instance, cell lines are used to investigate genetic mutations, cancer treatments, drug screening, aging, metabolism and vaccines. What Is a Cell Culture? Laboratory researchers culture large populations of cells from one or more parent cells.

What are the different types of cell lines?

Cell line Organism Origin Tissue HeLa Human Cervical cancer 293-T Human Kidney (embryonic) A-549 Human Lung carcinoma ALC Murine Bone marrow CHO Hamster Ovary HB54 Hybridoma Hybridoma FM3 Human Metastatic lymph node 26.

How are cell lines selected for their phenotype?

A given cell line contains several cell lineages of either similar or distinct phenotypes. It is possible to select a particular cell lineage by cloning or physical cell separation or some other selection method.

Which is the best description of a subcultured cell line?

Subcultured cells taken from a primary culture start a cell line. Unless otherwise altered, normal cells from a primary culture have a programmed life span, meaning they are finite. The strongest, fastest growing cells predominate and give rise to uniformity in the population.