What does HMB-45 stain?
What does HMB-45 stain?
HMB-45 is a widely used immunohistochemical stain for detection of primary as well as metastatic melanoma (1). Presumably, this method uses monoclonal antibodies to a glycoprotein that is present in premelanosomes (11).
What is mart1 stain?
Melan-A, also known as MART-1, is an additional melanocytic marker and has proved to be useful in identifying metastatic tumors of melanocytic origin. The usefulness of this marker to discriminate pigmented actinic keratosis from early melanoma in situ, however, has not yet been a subject of investigation.
What is s100 test?
High levels of S100A8/A9 (alias: calprotectin) and S100A12 (alias: Calgranulin C, EN-RAGE) are characteristic of active systemic juvenile idiopathic arthritis (sJIA) and may distinguish sJIA from other febrile illnesses, including systemic infection, various forms of leukemia, and Kawasaki disease.
Is melanoma S100 negative?
We emphasize that, albeit rare, malignant melanomas can be completely negative for S-100 protein and the use of additional melanocytic-associated markers in the differential workup maybe critical in arriving promptly at a proper diagnosis.
What is Melan A positive?
The presence of Melan-A positive dermal cells in excisions for melanoma in situ represents a frequent conundrum for pathologists. These cells may represent superficially invasive melanoma, benign, incidental, dermal nevi or non-specific staining of dermal melanophages.
What is CD68 positive?
CD68 In cancer. CD68 is broadly used as cancer-associated diagnostic and prognostic marker. It is used to identify neoplasms with macrophage lineage and also may be expressed by tumor cells from other lineages.
What is RMS illness?
Overview. Rhabdomyosarcoma (RMS) is a rare type of cancer that forms in soft tissue — specifically skeletal muscle tissue or sometimes hollow organs such as the bladder or uterus. RMS can occur at any age, but it most often affects children.
How is HMB-45 staining used in biopsy?
Biopsy specimens from 225 benign and malignant melanocytic lesions were examined after immunoperoxidase staining for S-100 protein and HMB-45.
Which is more intense HMB-45 or S-100?
S-100 and HMB-45 showed maximally intense staining in 50% and 54% of cases, respectively. S-100 and NK1/C3 stained both histiocytes and melanocytes, whereas MART-1 and HMB-45 stained only melanocytes.
What kind of lesions are positive for HMB-45?
Such lesions may be positive for HMB-45, or retest as positive using monoclonal and polyclonal antibodies against S100 protein.1933 S100 protein is also found in nevi and Langerhans cells, as well as cutaneous neural tumors.
Is the antibody HMB-45 used as a marker for melanoma?
The antibody HMB-45 used as an immunohistochemical reagent has often been labeled as a marker for melanoma, even though some benign lesions have been noted to show positive staining reactions with this reagent. Biopsy specimens from 225 benign and malignant melanocytic lesions were examined after im …
