What is adipocyte differentiation?
What is adipocyte differentiation?
Adipocyte differentiation is characterized by sequential changes in the expression of specific genes that determine the specific adipocyte phenotype of the cells. This is reflected by the appearance of various early, intermediate and late mRNA/protein markers and triglyceride accumulation.
What is the structure of adipocyte?
Each adipose cell consists of a large lipid droplet surrounded by a thin rim of cytoplasm. In addition to typical subcellular organelles, a variety of small lipid droplets and an extensive system of membranes characterize the cell’s cytoplasm. A fenestrated envelope surrounds the large, central lipid droplet.
What are the functions of adipose tissue?
The adipose tissue is a critical regulator of systemic energy homeostasis by acting as a caloric reservoir. In excess nutrient conditions, the adipose tissue stores surplus nutrients in the form of neutral lipids, whereas in nutrient deficit conditions, it supplies nutrients to other tissues through lipolysis (1).
How do you create differentiation media?
Formulating Complete Osteogenic Differentiation Media 1. Defrost 50mL of fetal bovine serum and 5mL of antibiotic/antimycotic solution in a 37°C water bath until ice in the tubes is no longer visible. 2. Immediately disinfect the tubes and the bottle containing the base media with 70% isopropranol.
How is Adipogenesis related to obesity?
However, adipocyte hyperplasia (adipogenesis) is now known to contribute to the increased adipose tissue mass of obesity. Animal studies suggest that hyperplasia occurs in 2 steps: an increase in numbers of preadipocytes and differentiation of preadipocytes into mature (adipokine-secreting) adipocytes.
Is body fat made of mucus?
Mucus is a protective substance released by various areas of the body such as the mouth, sinuses, throat, lungs, stomach, and intestines. Over 90% of mucus is water, but it also comprises fat, salts, proteins, some immune cells and mucins.
What is differentiation media?
Differentiation medium promotes cells to change from one type to another, often a less specialized type becoming more specialized in form and function, while Induction medium provides signals that change cell behavior, shape, differentiation, mitotic activity, signal cascades and/or gene expression.
What is the process of adipocyte differentiation called?
Adipose tissue growth involves an increase in adipocyte size and the formation of new adipocytes from precursor cells. For the last 20 years, the cellular and molecular mechanisms of adipocyte differentiation have been extensively studied using preadipocyte culture systems.
Can a stem cell be used for adipogenic differentiation?
Despite the growing body of research in obesity and adipose biology, MSC adipogenic differentiation is not restricted to endocrinologists. Stem cell scientists, bone biologists, and tissue engineering specialists all have a vested interest in the study of MSC adipogenesis.
What is the role of adipocyte in energy balance?
The adipocyte plays a critical role in energy balance. Adipose tissue growth involves an increase in adipocyte size and the formation of new adipocytes from precursor cells. For the last 20 years, the cellular and molecular mechanisms of adipocyte differentiation have been extensively studied using preadipocyte culture systems.
How is the growth and differentiation of preadipocytes controlled?
Growth and differentiation of preadipocytes is controlled by communication between individual cells or between cells and the extracellular environment. Various hormones and growth factors that affect adipocyte differentiation in a positive or negative manner have been identified.
