What is FuGENE 6 transfection reagent?

What is FuGENE 6 transfection reagent?

FuGENE® 6 Transfection Reagent(a) is a nonliposomal reagent that transfects DNA into a wide variety of cell lines with high efficiency and low toxicity. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex.

What is FuGENE transfection reagents and how does it work?

FuGENE® HD Transfection Reagent is a multi-component reagent that forms a complex with DNA, then transports the complex into animal or insect cells. It is suitable for use in media with or without serum, and for transient or stable transfection, as well as co-transfections of multiple DNA plasmids.

How does FuGENE work?

FuGENE® HD DNA Transfection FuGENE® HD interacts with nucleic acids to provide efficient and safe entry into the cell, allowing users to overcome barriers in difficult-to-transfect lines such as primary cells, stem cells, and suspension cells. Its simple, easy-to-use protocol allows users to free up valuable lab time.

How does TurboFect work?

TurboFect Reagent interacts with DNA to form small, stable, highly diffusible complexes which are readily endocytosed. The reagent creates a proton-sponge effect that buffers endosomal pH by provoking massive proton accumulation and passive chloride influx.

What is forward and reverse transfection?

Forward and reverse transfection protocols each have their significant uses in research. The main protocol difference between forward and reverse transfection is whether or not the cells are plated the day before transfection (as in forward transfection) or seeded at the same time of the transfection.

How do you know if transfection is working?

Determining the number of positive cells within a transfected cell population can be done through microscopy and flow cytometry. Finally, confirming localization of your protein of interest can be done by microscopy.

How can I increase my transfection efficiency?

Improving the Success of Your Transfection

1. Transfect healthy, actively dividing cells at a consistent cell density.
2. Transfect using high-quality DNA.
3. Optimize the amount of DNA used to transfect cells.
4. Optimize the transfection reagent:DNA ratio.
5. Optimize cell number per well when transfected.

How does the FuGene 6 transfection reagent protocol work?

FuGENE® 6 Transfection Reagent is a nonliposomal reagent that transfects DNA into a wide variety of cell lines with high efficiency and low toxicity. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex. Printed in USA. Revised 4/11.

Can you store FuGene 6 in a glass vial?

Do not freeze or store below 0°C. Formulation and Packaging: FuGENE® 6 Transfection Reagent is a proprietary mixture of lipids and other components in 80% ethanol, fi ltered through a 0.1µm fi lter and supplied in glass vials.

How big is the volume of FuGene 6?

not present 6:1 4:1 3:1 1.5:1 Medium to a fi nal volume of1 100µl 100µl 100µl 100µl DNA amount 2µg 2µg 2µg 2µg Volume of FuGENE® 6 Transfection Reagent 12µl 8µl 6µl 3µl

How big is a 1mL FuGene HD reagent?

The 1ml size contains sufficient reagent to transfect 333µg of DNA. This is equivalent to over 3,300 wells in. 96-well plates at a 3:1 FuGENE® HD Transfection Reagent:DNA ratio (0.3µl reagent:100ng DNA per well). The actual number of transfections will vary with reagent:DNA ratio, transfection volume and cell type.