What is Phenomenex column?

What is Phenomenex column?

Achieve Powerful HPLC Separations. LC MS analysis of Vitamin D2 and D3 from Serum. At Phenomenex, the goal is simple, to manufacture HPLC columns that give you the results that you expect: resolution, reproducibility, pH stability, method flexibility, selectivity, across any platform.

What is C18 2 column?

C18(2) Phase Information Luna C18(2) phase is an octadecyl silane with ligands bound to the silica surface,resulting in a C18 HPLC column with very hydrophobic stationary phase that offers great methylene selectivity.

Do HPLC columns expire?

Re: Will a new/unused HPLC column go bad over time? C18 columns in general are fairly robust, so it is *probably* OK. I generally run a “mini sysrem suitability” test on any new column, so that would tell the tale. If it fails, the age would certainly be ground for a return.

Are HPLC columns reusable?

Column reuse is an issue of both economics and chemistry. With reversed-phase chromatography, column reuse is a given. Why, because the hydrophobic stationary phase is washable with organic solvents to remove highly retained contaminants and the columns can be stored in acetonitrile or methanol for long time periods.

Why C18 column is used in HPLC?

C18 columns are HPLC (high performance liquid chromatography) columns that use a C18 substance as the stationary phase. C18 simply means that the molecules contain 18 carbon atoms, so the other atoms in the molecule can vary, leading to significantly different substances.

What is end cap column?

In chromatography, endcapping refers to the replacement of accessible silanol groups in a bonded stationary phase by trimethylsilyl groups. End-capped columns have much lower residual silanol group activity compared to non-endcapped columns.

What is the HPLC column?

Columns are the main component in HPLC because the column is responsible for the separation of the sample components. The sample passes through the column with the mobile phase and separates in its components when it comes out from the column. The material filled in the HPLC columns is known as a stationary phase.

How long does mobile phase last?

Some buffered or aqueous containing mobile phases will at most be good for one week at refrigerated temperatures (e.g. simple phosphate buffers), BUT some will still grow bugs in them during this time and be unsuitable for HPLC work.

How do I clean my HPLC columns?

5.0 PROCEDURE

1. Change the mobile phase to filtered distilled water.
2. Allow the water to flow through the column at the rate of 1ml / min.
3. Change the mobile phase from water to HPLC grade 80% Acetonitrile.
4. Set the instrument to flow rate 1 ml/ minute and wash the column for 30 minutes.

How do you clean cyano columns?

Wash the column with 5–10 mL of this — just enough to wash most of the buffer out. Then switch to 100% of the organic solvent, acetonitrile in this case. Further washing with 10–20 column volumes of the organic solvent should be sufficient to remove strongly retained materials.

Is C18 column polar or nonpolar?

A C18 column is an example of a “reverse phase” column. Reverse phase columns are often used with more polar solvents such as water, methanol or acetonitrile. The stationary phase is a nonpolar hydrocarbon, whereas the mobile phase is a polar liquid.

Which is the best Luna column for UHPLC?

Introducing Luna Omega. Enhanced with 20 Years of technology, innovation, and experience, Luna Omega 1.6µm columns build upon the Luna legacy to now provide incredible UHPLC performance and selectivity. With Luna Omega columns you gain: Greater separation muscle. Better peak shape through an inert foundation.

What should I use to clean my Luna HPLC column?

Reversed Phase • Apply the same gradient flush as in the cleaning above, overnight at low flow. Normal Phase • For water removal, flush with: 1. 30 mL 2.5% 2,2 Dimethoxypropane and 2.5% Glacial Acetic Acid in Hexane. 2. Then flush column with cleaning method from above, overnight at low flow rate.

What’s the maximum temp for a Luna column?

Continuous use of Luna columns at the maximum temperature limit may compromise column longevity. Expanded temperature use for Luna Omega phases based on common mobile phases: Formic acid: 90°C (C18, Polar C18), 80°C (PS C18) Trifluoroacetic acid: 80°C (C18, Polar C18), 60°C (PS C18)

Who is the manufacturer of the Luna column?

Phenomenex products are available worldwide. Each Luna column manufactured by Phenomenex is individually prepared and tested. Every column is supplied with a Certificate of Quality Assurance (CQA) which indicates testing conditions, operating parameters, and column details.