What is TagRFP?
What is TagRFP?
TagRFP is a basic (constitutively fluorescent) red fluorescent protein published in 2007, derived from Entacmaea quadricolor. It has very low acid sensitivity.
How does red fluorescent protein work?
Red fluorescent protein (RFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. RFP can be excited by the 488 nm or 532 nm laser line and is optimally detected at 588 nm.
What is Turbo RFP?
TurboRFP is a basic (constitutively fluorescent) orange fluorescent protein published in 2007, derived from Entacmaea quadricolor. It has low acid sensitivity.
Which other Colours of fluorescent protein exist?
GFP-like proteins are very diverse, as they can be not only green, but also blue, orange-red, far-red, cyan, and yellow. They also can have dual-color fluorescence (e.g., green and red) or be non-fluorescent.
How is GFP produced?
Gfp refers to the gene that produces green fluorescent protein. Using DNA recombinant technology, scientists combine the Gfp gene to a another gene that produces a protein that they want to study, and then they insert the complex into a cell.
How do you detect mCherry?
It’ll depend on the flow cytometer you have access to but to best detect the mCherry signal excite with the yellow-green laser at 561 nm and detect in the PE-TexasRed channel with a 610/20 bandpass filter.
What is the best red fluorescent protein?
mCherry is the most widely used and cited red fluorescent protein owing to its fast maturity, stability, and resistance to photobleaching. mCherry and DsRed-Monomer are ideal for tagging proteins with diverse functions and/or subcellular localization patterns.
What change to the amino acid sequence of green fluorescent protein helps it work more efficiently at 37 degrees?
Mutation of the phenylalanine residue at position 64 (Phe64) to leucine results in improved maturation of fluorescence at 37 degrees, which is at least equivalent to that observed at 28 degrees.
What is Turbo GFP?
TurboGFP is a basic (constitutively fluorescent) green fluorescent protein published in 2006, derived from Pontellina plumata. It is reported to be a rapidly-maturing dimer with moderate acid sensitivity.
What is RFP and GFP?
Green Fluorescent Protein (GFP) and Red Fluorescent Protein (RFP) are ~29 kDa fluorescent proteins used as versatile markers for visualization of protein localization, monitoring physiological processes, and for detecting transgenic or transient protein expression.
What are GFP like proteins?
GFP is a small, stable, and brightly fluorescent protein. A gene encoding GFP can be added to a cell and used to synthesize GFP, which then creates its own internal chromophore that fluoresces when illuminated with ultraviolet light, without consuming cellular energy.
Which proteins are fluorescent?
Table 1 – Fluorescent Protein Properties
| Protein (Acronym) | Excitation Maximum (nm) | Emission Maximum (nm) |
|---|---|---|
| Azurite | 384 | 450 |
| mTagBFP | 399 | 456 |
| Cyan Fluorescent Proteins | ||
| ECFP | 439 | 476 |
What kind of protein is the TagRFP protein?
TagRFP is a basic (constitutively fluorescent) red fluorescent protein published in 2007, derived from Entacmaea quadricolor. It has very low acid sensitivity.
Where does the N terminus of TagRFP come from?
Note: While the sequence below matches the sequence shown in Supp. Fig 1 of the original publication (Merzlyak et al, 2007), most commonly used variants (e.g. at Addgene and Snapgene) show TagRFP with N terminus from EGFP (MVSKGEE).
What can red fluorescent protein TagRFP-evrogen be used for?
It can also be used for cell and organelle labeling and for tracking the promoter activity.
What can TagRFP be used for in fluorescent dyes?
Cell lines expressing TagRFP are commercially available. TagRFP can be used in multicolor labeling applications with blue, cyan, green, yellow, and far-red fluorescent dyes. TagRFP is ideally suitable for use as an acceptor for FRET with Evrogen green fluorescent protein TagGFP2.
