What is the recognition sequence of Sau3AI?

What is the recognition sequence of Sau3AI?

The enzyme recognizes the palindromic sequence 5′-G(met-A,A)TC-3′ as determined by PEI chromatography of pancreatic DNase, snake venom phosphodiesterase digestion products of labelled fragments, analysis of restriction digests from normal and N6-methyladenine-free DNA and direct sequence analysis of cloned fragments.

What is degenerate recognition sequence?

For degenerate enzymes, any base represented by the single letter code may be present at either location in the recognition site for cleavage to occur. For example, BsrFI-v2 recognizes all of the following sequences: ACCGGC, ACCGGT, GCCGGC, GCCGGT.

What is the recognition sequence for TaqI?

The nucleotide sequence of the genes encoding methyltransferase TaqI (M. TaqI) and restriction endonuclease TaqI (R. TaqI) with the recognition sequence, TCGA, were analyzed in clones isolated from independent libraries. The genes, originally reported as 363 and 236 codons long [Slatko et al., Nucleic Acids Res.

Which type of restriction enzyme is sau3a1?

type II restriction endonuclease
Sau3AI, a monomeric type II restriction endonuclease that dimerizes on the DNA and thereby induces DNA loops.

What are Isoschizomers and Neoschizomers?

Isoschizomers are restriction enzymes that have the same recognition sequence and the same specificity. Neoschizomers recognize the same nucleotide sequence but cleave DNA at different positions.

How do restriction enzymes recognize a restriction site?

Each restriction enzyme recognizes a short, specific sequence of nucleotide bases (the four basic chemical subunits of the linear double-stranded DNA molecule—adenine, cytosine, thymine, and guanine). These regions are called recognition sequences, or recognition sites, and are randomly distributed throughout the DNA.

What is meant by recognition sequence?

A recognition sequence is a DNA sequence to which a structural motif of a DNA-binding domain exhibits binding specificity. Recognition sequences are palindromes. Depending on the degree of specificity of the protein, a DNA-binding protein can bind to more than one specific sequence.

What is a recognition sequence of a restriction enzyme?

Are the EcoRI ends sticky or blunt?

The ends of a molecule cut by EcoRI have an overhanging region of single stranded DNA, and so are sometimes called sticky-ends. On the other hand, EcoRV is an example of an enzyme that cuts both strands in exactly the middle of its recognition sequence, producing what are called blunt-ends, which lack overhangs.

Which two enzymes are examples of isoschizomers?

Isoschizomers are pairs of restriction enzymes specific to the same recognition sequence. For example, SphI (CGTAC/G) and BbuI (CGTAC/G) are isoschizomers of each other.

How many units of DNA are needed for Sau3AI?

One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl. Sau3AI and DpnII are isoschizomers of MboI. Blocked by overlapping CpG methylation.

What’s the difference between DpnII, dp3ai and Sau3AI?

One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl. Sau3AI and DpnII are isoschizomers of MboI. Blocked by overlapping CpG methylation. Why Choose Recombinant Enzymes? What’s the difference between DpnI, DpnII, MboI, and Sau3AI?

Where does BamHI bind in the recognition sequence?

BamHI binds at the recognition sequence 5′-GGATCC-3′, and cleaves these sequences just after the 5′-guanine on each strand. This cleavage results in sticky ends which are 4 bp long. In its unbound form, BamHI displays a central b sheet, which resides in between α-helices.

What is the recognition site for BAM H I?

The recognition site for Bam H I has a palindromic sequence which can be cut in half for ease in showing bonds. As of the end of 2010, there were 5 crystal structures of Bam H I in the Protein Data Bank BamHI, like other type II restriction endonucleases, often requires divalent metals as cofactors to catalyze DNA cleavage.